Seed and spread alpha-synuclein model

Time course of alpha-synuclein seeding and resulting loss of dopaminergic terminals after unilateral injection of sonicated murine pre-formed fibrils or PBS into the dorsal striatum. Stereotactic injection was performed at an age of 1.8 months. Pathological alpha-synuclein (pSer129-alpha-Syn) and loss of dopaminergic terminals (TH) were quantified 5, 9 and 13 weeks (control only at 9 weeks) after injection in the striatum as measured by IHC (mean ± SEM, n = 8). Statistics: Tukey’s One-Way ANOVA. Scale bar is 200 µm.

Amytracker 520 (Ebba Biotech) staining to pick up amyloidogenic structures 9 weeks post-injection in the dorsal striatum (left). The alpha-synuclein pathology spreads further to the amygdala (right). Amytracker 520 detect both Lewy Bodies and Lewy Neurites. Scale bar is 100 µm.

Time course of alpha-synuclein spreading to the contralateral striatum after injection of sonicated murine pre-formed fibrils or PBS into the ipsilateral dorsal striatum. Stereotactic injection was performed at an age of 1.8 months. Pathological alpha-synuclein (pSer129-alpha-Syn) was quantified 5, 9 and 13 weeks (control only at 9 weeks) after injection in the striatum as measured by IHC (mean ± SEM, n = 8). Statistics: Tukey’s One-Way ANOVA.