Seed and spread alpha-synuclein model

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  • Seed and spread alpha-synuclein model

Key characteristics

  • Unilateral stereotactic injection of sonicated mPFFs into the dorsal striatum
  • Succesful seeding and progressive spreading of alpha-synuclein pathology to other brain regions, including substantia nigra
  • Dopaminergic cell loss and microgliosis

Time course of alpha-synuclein seeding and resulting dopaminergic cell loss after unilateral injection of sonicated murine pre-formed fibrils or PBS into the dorsal striatum. Stereotactic injection was performed at an age of 1.8 months. Pathological alpha-synuclein (pSer129-alpha-Syn) and dopaminergic cell loss (TH) were quantified 5, 9 and 13 weeks (control only at 9 weeks) after injection in the striatum as measured by IHC (mean ± SEM, n = 8). Statistics: Tukey’s One-Way ANOVA.

Time course of alpha-synuclein spreading to the contralateral striatum after injection of sonicated murine pre-formed fibrils or PBS into the ipsilateral dorsal striatum. Stereotactic injection was performed at an age of 1.8 months. Pathological alpha-synuclein (pSer129-alpha-Syn) was quantified 5, 9 and 13 weeks (control only at 9 weeks) after injection in the striatum as measured by IHC (mean ± SEM, n = 8). Statistics: Tukey’s One-Way ANOVA.

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